Product name
EB Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Certificate
CE
Epidemiology
EBV (Epstein-barr virus), or human herpesvirus type 4, is a common human herpesvirus
[1]. In recent years, a large number of studies have proved that EBV is associated with occurrence and development of nasopharyngeal cancer, Hodgkin's disease, T/Natural killer celllymphoma, Burkitt's lymphoma, breast cancer, gastric cancer and other malignant tumors. And it is also closely associated with post-transplantlymphoproliferative disorders, post-transplant smooth muscle tumor and acquired immunedeficiency syndrome(AIDS) related lymphoma, multiple sclerosis, primary central nervous system lymphoma or leiomyosarcoma
[2-4].
Features
● Multiplex PCR Amplification Technology.
● Internal control: Fully monitor the experimental process to ensure the quality of Experiments.
● High sensitivity: 500Copies/mL.
● Convenient: One test for multiple results.
Channel
FAM: EBV
VIC (HEX): Internal control
PCR Amplification Conditions Setting
| Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
| 1 |
1 cycle |
50℃ |
2mins |
No |
| 2 |
1 cycle |
95℃ |
5mins |
No |
| 3 |
40 cycles |
95℃ |
15s |
No |
| 58℃ |
30s |
Yes |
Technical Parameters
| Storage |
≤-18℃ In dark |
| Shelf-life |
12 months |
| Specimen Type |
Whole blood, Plasma, Serum |
| Ct |
≤38 |
| CV |
≤5.0％ |
| LoD |
500Copies/mL |
Specificity: It has no cross-reactivity with other pathogens (such as human herpesvirus 1, 2, 3, 6, 7, 8, hepatitis B virus, cytomegalovirus, influenza A, etc.) or bacteria (Staphylococcus aureus, Candida albicans, etc.)
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
SLAN-96P Real-Time PCR Systems.
ABI 7500 Real-Time PCR Systems.
QuantStudio®5 Real-Time PCR Systems.
LightCycler®480 Real-Time PCR Systems.
LineGene 9600 Plus Real-Time PCR Detection Systems.
MA-6000 Real-Time Quantitative Thermal Cycler.
Main Components
| Catalogue Number |
Component (50 tests/kit) |
Specification |
Quantity |
Component Description |
| HWTS-OT061A |
EB Reaction Buffer |
1.175mL/vial |
1 vial |
Amplification reaction reagents, EB virus primer probes, internal reference primer probes, etc. |
| EB Enzyme Mix |
75μL/vial |
1 vial |
Taq enzyme, UDG enzyme, etc. |
| EB Positive Control |
600μL/vial |
1 vial |
Mixed solution of viral target gene and internal reference |
| EB Blank Control |
600μL/vial |
1 vial |
DNase/RNase free H2O |
Total PCR Solution
Reference
[1] ohen JI, Jaffe ES, Dale JK, et al. Characterization and treatment of chronic active Epstein-Barr virus disease: a 28-year experience in the United States[J]. Blood, 2011, 117(22): 5835-5849.
[2] Jahangiryan A, Kheirandish M, Samiee S, et al. Determination of Epstein-Barr virus (EBV) incidence in umbilical cord blood (UCB) and assessment of virus DNA via Real-time PCR[J]. Annals of Cancer Research and Therapy, 2021, 29(1):114-120.
[3] Petersson F. Nasopharyngeal carcinoma:a review[J].Semin Diagn Pathol, 2015, 32(1):54-73. [4]Chen J N, He D, Tang F, et al. Epstein-Barr virus-associated gastric carcinoma:a newly defined entity[J]. J Clin Gastroenterol, 2012, 46(4):262-271.