Neisseria Gonorrhoeae Nucleic Acid

Product Name

HWTS-UR003A-Neisseria Gonorrhoeae Nucleic Acid Detection Kit  (Fluorescence PCR)

Epidemiology

Gonorrhea is a classic sexually transmitted disease caused by infection with Neisseria gonorrhoeae (NG), which mainly manifests as purulent inflammation of the mucous membranes of the genitourinary system. NG can be divided into several ST types. NG can invade the genitourinary system and reproduce, causing urethritis in men, urethritis and cervicitis in women. If not treated thoroughly, it can spread to the reproductive system. The fetus can be infected through the birth canal resulting in neonatal gonorrhea acute conjunctivitis. Humans have no natural immunity to NG and are susceptible to NG. Individuals have weak immunity after infection which cannot prevent reinfection.

Channel

FAM: NG target·

VIC(HEX): Internal Control

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	5mins	No
2	1 cycle	95℃	10mins	No
3	40 cycles	95℃	15 secs	No
4		58℃	31 secs	Yes

PCR Amplification Conditions Setting

Storage
Liquid	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Male urethral secretions, Male urine, Female exocervical secretions
Ct	≤38
CV	≤5.0%
LoD	50Copies/reaction
Specificity	There is no cross-reactivity with other STD pathogens, such as Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and etc.
Specificity	There is no cross-reactivity with other STD pathogens, such as Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and etc.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR System QuantStudio® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1. Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006). Option 2. Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.