Product name
Hepatitis B Virus Genotyping Detection Kit (Fluorescent PCR)
Packaging Size
50 tests/kit
Intended Use
This kit is used for qualitative typing detection of type B, type C and type D in the positive serum/plasma samples of hepatitis B virus (HBV).
Epidemiology
At present, ten genotypes from A to J of HBV have been identified worldwide. Different HBV genotypes have differences in epidemiological characteristics, virus variation, disease manifestations and treatment response, etc., which will affect the HBeAg seroconversion rate, the severity of liver lesions, and the incidence of liver cancer to a certain extent, and affect the clinical prognosis of HBV infection and the therapeutic efficacy of antiviral drugs to a certain extent.
Features
● Multiplex PCR Amplification Technology
● Internal control: Fully monitor the experimental process to ensure the quality of experiments.
● High sensitivity: 1×10
2 IU/mL
● High specificity: No cross-reactivity with common pathogens for more accurate results.
Channel
| Channel
Name |
Reaction Buffer 1 |
Reaction Buffer 2 |
| FAM |
HBV-C |
HBV-D |
| VIC/HEX |
HBV-B |
Internal Control |
PCR Amplification Conditions
| Step |
Cycles |
Temperature |
Time |
Collect Fluorescent Signals or Not |
| 1 |
1 cycle |
50℃ |
2 mins |
No |
| 95℃ |
5 mins |
No |
| 2 |
40 cycles |
95℃ |
15 secs |
No |
| 60℃ |
45 secs |
Yes |
Technical Parameters
Storage: ≤-18℃ In dark
Shelf-life: 12 months
Specimen Type: Serum, Plasma
Ct: ≤38
CV: ≤5.0％
LoD: 1×10
2 IU/mL
Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market.
ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
QuantStudio®5 Real-Time PCR Systems
LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
MA-6000 Real-Time Quantitative Thermal Cycler
BioRad CFX96 Real-Time PCR System
BioRad CFX Opus 96 Real-Time PCR System
Main Components
|
Catalogue Number
|
Component (50 tests/kit)
|
Specification
|
Quantity
|
Component Description
|
|
HWTS-HP002A
|
HBV-FX Reaction Buffer 1
|
1mL/vial
|
1 vial
|
Prepared in a certain proportion with primers and probes of HBV Types B and C, PCR Mix, UNG enzyme.
|
|
HBV-FX Reaction Buffer 2
|
1mL/vial
|
1 vial
|
Prepared in a certain proportion with primers and probes of HBV Type D and internal control, PCR Mix, UNG enzyme
|
|
HBV-FX Positive Control
|
1mL/vial
|
1 vial
|
Inactivated human serum containing HBV types B, C and D.
|
|
HBV-FX Negative Control
|
1mL/vial
|
1 vial
|
Inactivated HBV-free human serum Please refer to the set value in the kit for specific concentration.
|
|
HBV-FX Internal Control
|
50μL/vial
|
1 vial
|
Self-made internal control plasmid
|
[References]
[1] Wang Z, Tanaka Y, Huang Y, et al. Clinical and Virological Characteristics of Hepatitis B Virus Subgenotypes Ba, C1, and C2 in China[J]. American Society for Microbiology, 2007(5).
[2] Yuen M F, Tanaka Y, Shinkai N, et al. Risk for hepatocellular carcinoma with respect to hepatitis B virus genotypes B/C, specific mutations of enhancer II/core promoter/precore regions and HBV DNA levels[J]. Gut, 2008, 57(1):98-102.
[3] Osiowy C, Giles E. Evaluation of the INNO-LiPA HBV Genotyping Assay for Determination of Hepatitis B Virus Genotype[J]. Journal of Clinical Microbiology, 2003, 41(12):5473-7.
[4] Furusyo N, Nakashima H, Kashiwagi K, et al. Clinical outcomes of hepatitis B virus (HBV) genotypes B and C in Japanese patients with chronic HBV infection.[J]. American Journal of Tropical Medicine & Hygiene, 2002, 67(2):151-157.