Herpes Simplex Virus Type 2 Nucleic Acid

Product Name

HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)

Intended Use

This kit is used for qualitative detection of herpes simplex virus type 2 nucleic acid in male urethral swab and female cervical swab samples.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) is a circular virus synthesized with tegument, capsid, core, and envelope, and contains double-stranded linear DNA. Herpes virus can enter the body through direct contact or sexual contact with skin and mucous membranes, and is divided into primary and recurrent. Reproductive tract infection is mainly caused by HSV2, male patients are manifested as penile ulcers, and female patients are manifested as cervical, vulvar, and vaginal ulcers. The initial infections of genital herpes virus are mostly recessive infections, except for a few local herpes with mucous membranes or skin, most of which have no obvious clinical symptoms. Genital herpes infection has the characteristics of lifelong virus carrying and easy recurrence, and both patients and carriers are the source of infection of the disease. In China, the serological positive rate of HSV2 is about 10.80% to 23.56%. The stage of HSV2 infection can be divided into primary infection and recurrent infection, and about 60% of HSV2 infected patients relapse.

Epidemiology

FAM: Herpes Simplex Virus Type 2 (HSV2)·

VIC(HEX): Internal Control

 

PCR Amplification Conditions Setting

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 Cycle	50℃	5mins	No
2	1 Cycle	95℃	10mins	No
3	40 Cycles	95℃	15secs	No
4		58℃	31secs	Yes

Technical Parameters

Storage
Liquid	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Female cervical swab, Male urethral swab
Ct	≤38
CV	≤5.0%
LoD	50Copies/reaction
Specificity	There is no cross-reactivity with other STD pathogens, such as Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and etc.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System.

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.