Respiratory Pathogens Combined

Product name

HWTS-UR106A-Respiratory Pathogens Combined Detection Kit(Fluorescence PCR)

Epidemiology

Diseases caused by pathogenic microorganisms invading the human nasal cavity, throat, trachea, bronchus, lungs and other respiratory tissues and organs and multiplying are called respiratory tract infections. Clinical manifestations include fever, cough, runny nose, sore throat, general fatigue and soreness . Respiratory pathogens include viruses, mycoplasma, chlamydia, bacteria, etc. Most of the infections are caused by viruses. Respiratory pathogens have the following characters such as many kinds of types, rapid evolution, complex subtypes, similar clinical symptoms. It has the clinical characters like the rapid onset, rapid spread, strong infectivity, and similar symptoms that are difficult to distinguish, which poses a serious threat to human health.

Channel

FAM	IFV A, IFV B Victoria, PIV type 1, hMPV type 2, ADV, RSV type A, MV·
VIC(HEX)	IFV B, H1, IFV B Yamagata, Internal reference
CY5	Internal reference, PIV type 3, hMPV type1, RSV type B
ROX	Internal reference, H3, PIV type 2

Technical Parameters

Storage	Liquid: ≤-18℃ In dark
Shelf-life	9 months
Specimen Type	Freshly collected oropharyngeal swabs
Ct	≤38
CV	≤5.0%
LoD	500Copies/mL
Specificity	There is no cross-reactivity with human genome and other respiratory pathogens.
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Option 2.

Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.