Hepatitis C Virus RNA Nucleic Acid

Product Detail

Product name

Hepatitis C Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)

Packaging Size

50 tests/kit

Intended Use

The HCV Quantitative Real-Time PCR Kit is an in vitro Nucleic Acid Test (NAT) to detect and quantitate Hepatitis C Virus (HCV) nucleic acids in human blood plasma or serum samples with an aid of Quantitative Real-Time Polymerase Chain Reaction (qPCR) method.

Epidemiology

The hepatitis C virus (HCV) is a small, enveloped, single-stranded, positive-sense RNA virus. HCV is spread primarily by direct contact with human blood. It is a major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer.

Features

● Multiplex PCR Amplification Technology ● Internal control: Fully monitor the experimental process to ensure the quality of experiments ● High sensitivity: 50IU/mL ● High specificity: No cross-reactivity with common pathogens for more accurate results

Channel

FAM: HCV RNA VIC (HEX): Internal control

PCR Amplification Conditions

Step	Cycles	Temperature	Time	Collect Fluorescent Signals or Not
1	1 cycle	50℃	15 mins	No
1	1 cycle	95℃	10 mins	No
2	5 cycles	95℃	15 secs	No
2	5 cycles	55℃	45 secs	No
3	40 cycles	95℃	15 secs	No
3	40 cycles	55℃	45 secs	Yes

Technical Parameters

Storage: ≤-18℃ In dark Shelf-life: 9 months Specimen Type: Serum, Plasma Ct: ≤36 CV: ≤5.0％ LoD: 50IU/mL Applicable Instruments: It can match the mainstream fluorescent PCR instruments on the market. ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems SLAN-96P Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-HP003A	HCV Reaction Buffer	1.425mL/vial	1 vial	Reaction Buffer,Primers and probes of HCV and Internal control
	HCV Enzyme Mix	75μL/vial	1 vial	Reverse transcriptase, etc.
	HCV Internal Control (IC)	50μL/vial	1 vial	Construction of pseudovirus solution in vitro
	HCV Quantitation Standard (QS) 1	0.7mL/vial	1 vial	HCV Pseudo-virus at defined concentration
	HCV QS 2	0.7mL/vial	1 vial	HCV Pseudo-virus at defined concentration
	HCV QS 3	0.7mL/vial	1 vial	HCV Pseudo-virus at defined concentration
	HCV QS 4	0.7mL/vial	1 vial	HCV Pseudo-virus at defined concentration
	HCV Positive Control	0.7mL/vial	1 vial	In vitro developed HCV pseudovirus solution
	HCV Negative Control	0.7mL/vial	1 vial	DNase/RNase free H₂O

[References] [1] I. Casas, L. Powell, P.E. Klapper, G.M. Cleator. New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay. Journal of Virological Methods. 1995. [2] K.H. Heermann,H. Seitz, R. Thomssen. Capture and RT-PCR of hepatitis C virus RNA with safety primers. Journal of Virological Methods. 1996. [3] Frederick S. Nolte, Cathy Thurmond, and P. Shawn Mitchell. Isolation of Hepatitis C Virus RNA from Serum for Reverse Transcription-PCR. J. Clin. Microbiol. 1994.