Herpes Simplex Virus Type 2 Nucleic Acid

Product Name

Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Certificate

CE

Intended Use

This kit is used for the qualitative detection of herpes simplex virus type 2 nucleic acid in genitourinary tract samples in vitro.

Epidemiology

Herpes Simplex Virus Type 2 (HSV2) is a circular virus synthesized with envelope, capsid, core, and envelope, and contains double-stranded linear DNA. Herpes virus can enter the body through direct contact with skin and mucous membranes or sexual contact, and is divided into primary and recurrent. Reproductive tract infection is mainly caused by HSV2, male patients manifest as penile ulcers, and female patients is cervical, vulvar, and vaginal ulcers. The initial infection of genital herpes virus is mostly a recessive infection. Except for a few herpes in mucous membranes or skin, most of them have no obvious clinical symptoms. Genital herpes infection has the characteristics of life-long and easy recurrence.Both patients and carriers are the source of infection of the disease.

Features

Internal control: Internal and external quality control to avoid false positive and false negative results.

High sensitivity: 400Copies/mL

High specificity: No cross-reactivity with other genitourinary tract infection pathogens and human genomic DNA

Channel

FAM: HSV2 nucleic acid·

ROX: Internal Control

Real Time Isothermal Amplification

Step	Temperature	Duration	Cycle	Fluorescent signal measurement
1	63℃	1min	30 cycles	Yes

 

Technical Parameters

Storage
Liquid	≤-18℃ In dark
Shelf-life	9 months
Specimen Type	Female cervical swab、Male urethral swab
Tt	≤28
CV	≤10.0%
LoD	400Copies/mL
Specificity	No cross-reactivity between this kit and other genitourinary tract infection pathogens, such as High-risk HPV 16, HPV 18, Treponema pallidum, Herpes simplex virus type 1, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, HIV virus, Lactobacillus casei and human genomic DNA.
Applicable Instruments	Applied Biosystems 7500 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR system, Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS1600).

Main Components

Catalogue Number	Component (50 tests/kit)	Specification	Quantity	Component Description
HWTS-UR025A	HSV2 Reaction Buffer	1.1mL/vial	1vial	Constant temperature amplification reaction Buffer, dNTP, DNase/RNase free H2O and primers, etc.
	HSV2 Enzyme Mix	150µL/vial	1vial	Constant temperature amplification Bst enzyme and probe, RNaseH, etc.
	HSV2 Positive Control	1mL/vial	1vial	Target gene recombinant plasmid
	HSV2 Internal Control	300µL/vial	1vial	Internal control gene recombinant plasmid
	HSV2 Negative Control	1mL/vial	1vial	DNase/RNase free H2O

Work Flow

1. Sample Collection Female cervical swab: wash the external cervical secretions with a sterile saline cotton ball, then insert a sterile cotton ball swab into the cervix, and then rotate the cotton swab for 5 seconds to collect cervical secretions. Place the swab in a sterile sample tube, close the tube cap, and seal it for testing. Male urethral swab: Do not urinate within 2 hours before collecting the sample, use a small cotton swab to insert into the urethra for about 2-4cm, and twist the swab to collect secretions. Place the swab in a sterile sample tube, close the tube cap, and seal it for testing. 2. Sample Process Add 1mL of sterile saline to the sterile tube containing the swab sample, shake and mix well, squeeze the cotton swab dry, and discard it.   Option 1. Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Option 2. Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

References

[1] Wagner, E. K., Sandri-Goldin, R. M. Herpes simplex viruses: molecular biology. in Encyclopedia of Virology (eds Brian W. J. M. & Marc H. V. V. R.) 397–405 (2008). [2] Corey L, Adams HG, Brown ZA, Holmes KK. Genital herpes simplex virus infections: clinical manifestations, course, and complications. Ann Intern Med. 1983 Jun;98(6):958-72. [3] Li JM, Chen YR, Li XT, Xu WC. Screening of Herpes simplex virus 2 infection among pregnant women in southern China. J Dermatol. 2011 Feb;38(2):120-4.