Mycobacterium Tuberculosis INH Resistance

Product Name

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)

Epidemiology

Isoniazid, a key anti-tuberculosis drug introduced in 1952, is one of the most effective drugs for combined treatment of active tuberculosis and a single drug for latent tuberculosis. KatG is the main gene encoding catalase-peroxidase and katG gene mutation can promote the synthesis of mycolic acid cell wall, making the bacteria resistant to isoniazid. KatG expression is negatively correlated with changes in INH-MIC, and a 2-fold decrease in katG expression results in a slightly larger 2-fold increase in MIC. Another cause of isoniazid resistance in mycobacterium tuberculosis occurs when base insertion, deletion or mutation occurs in the InhA gene locus of mycobacterium tuberculosis.

Channel

ROX	inhA (-15C>T) site·
CY5	katG (315G>C) site
VIC (HEX)	IS6110

Technical Parameters

Storage	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	Sputum
CV	≤5.0%
LoD	1 × 103 bacteria/mL
Specificity	No-cross reactivity with the mutations of the four drug resistance sites (511, 516, 526 and 531) of the rpoB gene outside the detection range of the detection kit. Applicable Instruments: Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

Work Flow

Option 1.

Recommended extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).

Option 2.

Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) and Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).