Ureaplasma Urealyticum Nucleic Acid

Product Name

HWTS-UR002A-Ureaplasma Urealyticum Nucleic Acid Detection Kit(Fluorescence PCR)

Intended Use

This kit is suitable for the qualitative detection of Ureaplasma urealyticum (UU) in male urinary tract and female genital tract secretion samples in vitro.

Epidemiology

The most common disease caused by Ureaplasma urealyticum is non-gonococcal urethritis, accounting for 60% of non-bacterial urethritis. Ureaplasma urealyticum parasites in male urethra, penis foreskin and female vagina. Ureaplasma urealyticum can cause urinary tract infections and infertility under certain conditions. If infected, it can cause prostatitis or epididymitis in men, vaginitis, cervicitis in women, and can infect the fetus leading to miscarriage, premature birth and low birth weight fetus, and can also cause infection of the neonatal respiratory tract and central nervous system.

Channel

FAM: UU nucleic acid·

VIC(HEX): Internal Control

PCR Amplification Conditions Setting

Step	Temperature	Time	Cycles	Collect Fluorescent Signals or Not
1	50℃	5mins	1 cycle	No
2	95℃	10mins	1 cycle	No
3	95℃	15 secs	40 cycles	No
4	58℃	31 secs		Yes

Technical Parameters

Storage
Liquid	≤-18℃ In dark
Shelf-life	12 months
Specimen Type	urethral secretions, cervical secretions
Ct	≤38
CV	≤5.0%
LoD	50Copies/reaction
Specificity	There is no cross-reactivity with other STD infection pathogens outside the detection range of the kit, such as Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, herpes simplex virus type 1, and herpes simplex virus type
Applicable Instruments	It can match the mainstream fluorescent PCR instruments on the market. Applied Biosystems 7500 Real-Time PCR Systems QuantStudio® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System

 

Work Flow

Option 1. Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) and Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006). Option 2. Recommended extraction reagent: Nucleic Acid Extraction or Purification Reagent(YDP302) by Tiangen Biotech(Beijing) Co.,Ltd.